plasmid-based construct

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• Plasmid stabilisation technology — Introduction In Molecular Biology and Biotechnology, Plasmid stabilisation technologies are an important issue in particular for the production of recombinant proteins in procaryotic hosts. Technologies using recombinant DNA have become of… …   Wikipedia

• DNA vaccination — The making of a DNA vaccine. DNA vaccination is a technique for protecting an organism against disease by injecting it with genetically engineered DNA to produce an immunological response. Nucleic acid vaccines are still experimental, and have… …   Wikipedia

• Two-hybrid screening — Overview of two hybrid assay, checking for interactions between two proteins, called here Bait and Prey . A. Gal4 transcription factor gene produces two domain protein ( BD and AD ) which is essential for transcription of the reporter gene ( LacZ …   Wikipedia

• Life Sciences — ▪ 2009 Introduction Zoology       In 2008 several zoological studies provided new insights into how species life history traits (such as the timing of reproduction or the length of life of adult individuals) are derived in part as responses to… …   Universalium

• Bacterial artificial chromosome — A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F plasmid), used for transforming and cloning in bacteria, usually E. coli.[1][2] F plasmids play a crucial role because they contain… …   Wikipedia

• Paired-end Tags — Paired end tags, also known as PET, refer to the short sequences at the 5’ and 3’ ends of the DNA fragment of interest, which can be a piece of genomic DNA or cDNA. These short sequences are called tags or signatures because, in theory, they… …   Wikipedia

• Synthetic biology — is a new area of biological research that combines science and engineering. It encompasses a variety of different approaches, methodologies, and disciplines with a variety of definitions. What they all have in common, however, is that they see… …   Wikipedia

• Gene therapy — using an Adenovirus vector. A new gene is inserted into an adenovirull. If the treatment is successful, the new gene will make a functional protein. Gene therapy is the insertion, alteration, or removal of genes within an individual s cells and… …   Wikipedia

• Gene synthesis — is the process of synthesizing an artificially designed gene into a physical DNA sequence.Gene synthesis was first demonstrated by Har Gobind Khorana in 1970 for a short artificial gene. Nowadays, commercial gene synthesis services are available… …   Wikipedia

• Molecular cloning — refers to a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms.[1] The use of the word cloning refers to the fact that the method involves the …   Wikipedia

• DNA — For a non technical introduction to the topic, see Introduction to genetics. For other uses, see DNA (disambiguation). The structure of the DNA double helix. The atoms in the structure are colour coded by element and the detailed structure of two …   Wikipedia